Computer-aided quantification of tumor infiltrating lymphocytes in standard immunohistochemical slides.

Non-profit free medical software.

Ver. 05.01 (final release) - October 18, 2019

Tumor-infiltrating lymphocytes assessment Oncoimmunoquantifier

     The quantitative evaluation of tumor infiltrating lymphocytes (TILs) can provide useful data on the prognosis of solid tumors and their metastases, but few surgical pathology laboratories have a specific image analysis system for computer-aided quantification of TILs in standard immunohistochemical slides. However, almost all laboratories are potentially able to perform such an analysis, having slide scanners and/or microscopes capable of capturing images on a computer. On the other hand, the microscopic quantification of TILs in routine hematoxylin and eosin (H&E) slides, although carefully standardized, will hardly achieve the same objectivity and reproducibility of a computerized analysis of immunohistochemical stains that can also quantify the specific lymphocyte populations involved in infiltration (CD8, CD4, and so on) considering the heterogeneity of TILs. For these reasons we developed OncoImmunoQuantifier (OIQ), a non-profit free Windows app that automatically quantifies TILs in standard digital images of immunohistochemical slides, allowing the spread of this evaluation without costs. Furthermore, it is possible to use OIQ even if the image capture system is based on a computer with an operating system different from Windows, since the app can also work on MacOS, Linux or other operating systems by installing Windows on Oracle VM VirtualBox, or using Boot Camp, Wine or Parallels Desktop.

  OIQ manages sequences of standard digital images captured from immunohistochemical slides by you with the software of your system (note that the app does not capture images by itself). Therefore you are not tied to a particular hardware. All you need is a slide scanner or a microscope equipped with a digital camera. OIQ get automatically the density of TILs (number of TILs per mm square of tissue) saving the results in Excel or Text file format. Moreover, the app can score density according to mean, median, tertiles, and quartiles. Other indices can be easily obtained based on literature data or according to your particular needs. In case of using a slide scanner, the software of the scanner must allow to select areas of fixed or variable size within the whole slide image and save them in standard graphics files.  A useful feature of the app is the ability to process images captured at low microscopic magnification (4x or 5x) to evaluate large areas of the slide in order to obtain more significant results.

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